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Department of Biological Sciences Optical Facility Optical Facility The following is a description of the systems (resources or equipment) available in the Facility:  A Bio-Rad MRC 1024 Scanning Confocal (3 channel/LaserSharp 2000 version 5.0 program) System, utilizing a Krypton-Argon (Kr-Ar) laser with excitation filters for 488 nm, 568 nm, 647 nm, 488 +568 nm (double staining) and all lines of a Kr-Ar laser for fluorescence confocal microscopy. It uses the following filters to detect the wavelengths of fluorophores with emission curves ranging from 573 nm to 637 nm (TRITC/Rhodamine), 487 nm to 557 nm (FITC/GFP) and 648 nm to 712 nm (far-red regions). It is attached to a Nikon Diaphot 200 inverted microscope using 100-watt mercury bulb for fluorescence imaging, and has the following objective lenses: a Plan 10x dry lens, and 4 Plan Apo lens which are a 20x dry, 40x oil, 60x oil, and 100x oil. The System is maintained by a yearly service contract with Carl Zeiss MicroImaging, and the LaserSharp program software is upgraded to current versions as they come on line. There is a user's fee to use this system. A Leica TCS SP2 (True Confocal Scanner) by Leica Microsystems, which utilizes 4 lasers (1 Argon-Krypton, and 3 Helium-Neon),
An Hitachi H-600 Transmission Electron Microscope that is maintained by a yearly service contract, with a routine overhaul preformed
The Facility has a fully set up Darkroom for black and white film and paper processing. Associated equipment consists of seal presses for journal article plates/poster session presentations and a large paper cutter for cutting poster board. The Facility also has a Stand alone, networked, digital processing station, with scanner. It uses Adobe PhotoShop for image processing and has a Codonics (Kodak) dye sublimation printer with both color and black and white formats attached to it.
Facility Manager: Committee Chairman: Kevin Vaughan Committee Members: Kevin Vaughan, Edward Hinchcliffe, David Hyde, Kenneth Olson, Jeffrey Schorey, Frank Collins, Crislyn D'Souza-Schorey, Bill Archer The Optical Facility has a number of optical data acquiring/processing systems and associated equipment used for specimen preparations. A full-time Lab manager/technician who assists in research projects, training, maintenance, and inventory maintains the Facility. |
allowing the users to choose from 8 excitation wavelengths (458, 476, 488, 496, 514, 543, 594, 633). Instead of using filter sets to detect the excited fluorophores emissions, it uses its exclusive AOBS (Acousto-Optical Beam Splitter) crystal to separate the emissions wavelengths coming from the samples. In part it functions like a spectrometer. This allows the users to fine tune the PMT's on what they are detecting, which in turn helps to reduce bleed through from one PMT to another. The system is attached to a Leica DM IRE2 automated inverted microscope using a 100 watt mercury bulb for fluorescence imaging, and the following objective lenses: a Plan Fluotar 20x dry lens, and 4 Plan Apo lenses which are a 20x multi-media (oil, water, or glycerol), 40x oil, 63x oil, and 100x oil. It also has the polarizing filters and prisms for DIC (Differential Interference Contrast) imaging. The system is maintained by a yearly service contract with Leica, and the Leica Confocal Software is updated to current versions as they come on line. There is a user's fee to use this system. 
The Metamorph/Metafluor Imaging System, by Universal Imaging, for both bright-field and fluorescence microscopy. It utilizes a Roper Scientific CoolSNAP ES CCD (charged-coupled device) camera to acquire images, and is attached to a Zeiss Axiovert S100 TV inverted microscope. The fluorescence capability is by either 75-watt xenon or 100-watt mercury bulb. It has the following objective lenses: A-Plan 5x dry, CP-ACHROMAT10x dry, Plan-NEOFLUAR 20x dry, 40x oil, 63x oil, and 100x oil. It also, has the following excitation filter sets; 340 nm (UV range), 380 nm (UV range), 420 nm (DAPI), 488 nm (FITC/Fluorescein), 555 nm (TRITC/Rhodamine), and a triple filter (420/488/555 nm) for triple staining procedures. The System is networked and the current version software from Universal Imaging is 5.01. Associated with this System is a stand alone, networked, processing station with the Metamorph/Metafluor software for further image processing.
yearly. Associated equipment used in specimen preparation for TEM microscopy, includes a LKB Knifemaker (for production of glass knives), 2 Sorvall MT-2 Ultramicrotomes (for plastic sectioning), and a RMC MT-X Ultramicrotome (for both plastic and frozen sectioning). A user’s fee (Beam Time) is charged for use of the scope.
A JEOL JSM T-300 Scanning Electron Microscope. Associated equipment used in specimen preparation for SEM microscopy, includes systems by Denton Vacuum, which are a CPD-1 Critical Point Dryer, a DeskII cold/etch Sputter Coater, and a DV-502A Vacuum Evaporator. A user fee (Beam Time) is charged for use of the scope.
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